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对宁夏地区2011年分离到的5株H1N1亚型猪流感病毒进行了基因组测定和遗传进化分析。结果显示:宁夏地区H1N1亚型猪流感病毒具有多样性,5株分离株可分为3类,其中1株为类人H1N1猪流感病毒,2株为经典猪流感病毒,另外2株为重组猪流感病毒,其PB2、PB1、PA、NP、NS基因来源于北美三元重组猪流感病毒,HA、NA、M基因来源于经典猪流感病毒;HA蛋白裂解位点附近氨基酸分析显示5株H1N1亚型猪流感分离株均为低致病性毒株;序列分析结果显示5株病毒在HA蛋白受体结合位点、抗原位点,以及NA蛋白潜在糖基化位点处氨基酸存在差异,这些差异具有分支特异性。5株病毒在NA和M2蛋白上均未出现与神经氨酸酶抑制剂和金刚烷胺耐药性相关的氨基酸变异,但其中3株病毒的PB2蛋白基因具有哺乳动物适应性变异E627K。
Five genotypes of H1N1 swine influenza virus isolated from Ningxia in 2011 were genomically analyzed and analyzed. The results showed that the H1N1 subtype swine influenza virus in Ningxia was diversified. Five isolates could be divided into three groups, of which one was humanoid H1N1 swine influenza virus, two were classical swine influenza virus and the other two were recombinant swine influenza virus Influenza virus, PB2, PB1, PA, NP, NS genes from North American ternary recombinant swine influenza virus, HA, NA, M genes derived from classical swine influenza virus; amino acid analysis near the HA protein cleavage site showed that five strains of H1N1 Type swine influenza isolates were all low-virulent strains. Sequence analysis showed that the five viruses showed differences in amino acid binding sites, antigenic sites and potential glycosylation sites of NA protein in HA protein receptor. These differences With branch specificity. The five viruses showed no amino acid mutations associated with neuraminidase inhibitors and amantadine resistance on NA and M2 proteins, but the PB2 protein gene of three viruses had a mammalian adaptive mutation E627K.