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建立白色念珠菌的生物分型法 ,并探讨其型别与毒力的关系。方法 临床标本直接接种在生物形态分型培养基上 ,30℃烛缸或 CO2 培养箱孵育 5 d,立即观察条状菌苔周围的条纹和色素环特征 ,并用 4个数字组成的编码记录型别。这 4个数字分别依次表示条纹分布、宽度、质地和色素环宽度。结果 该法把 70 0株临床分离白色念珠菌分成 6 9个型 ,以 0 0 0 2型 (2 0 .6 % )为最常见 ,分辨指数为 0 .931;红褐色色素环可能是白色念珠菌所特有的 ,而且不连续条纹与白色念珠菌的毒力相关。结论 生物分型法具有简单、经济、重复性好和分辨率高等优点 ,可作为一种有用的流行病学调查的工具以预防和监测白色念珠菌感染 ,尤其医院感染 ,而且它的型别可以指示白色念珠菌毒力。
The establishment of Candida albicans bioassay, and explore the relationship between its type and virulence. Methods The clinical specimens were directly inoculated onto the biological morphological medium and incubated at 30 ℃ in a candle or CO2 incubator for 5 days. The streaks and pigment ring features around the streaky bacteria were observed immediately. The 4-digit coded record type . The four numbers represent the stripe distribution, width, texture, and pigment ring width, respectively. Results The 70 0 strains of clinical isolates of Candida albicans were divided into 6 9 types. The type 0 0 0 2 (20.6%) was the most common, with a discrimination index of 0. 931. The reddish-brown pigment ring could be white rosary The fungus is endemic, and the discontinuous streaks are associated with C. albicans virulence. Conclusions Biophenotyping has the advantages of simplicity, economy, good reproducibility, and high resolution and can be used as a useful tool for epidemiological investigations to prevent and monitor Candida albicans infections, especially nosocomial infections, and its type can Candida albicans toxicity.