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目的了解2013年-2016年湖州市禽类环境中禽流感病毒污染情况,评估人感染禽流感风险。方法采集禽类饮用水等环境标本,采用荧光RT-PCR对标本进行甲型流感病毒核酸检测,阳性标本再检测H5、H7、H9亚型禽流感病毒核酸。结果 2013年-2016年共采集并检测各类环境标本5 867份,甲型流感病毒总阳性率为38.5%,检出的禽流感病毒型别以H7亚型为主(占23.6%)。流感病毒检测阳性率高峰呈现于冬春季节;且阳性率较高的分别为城乡活禽市场(77.8%)、家禽屠宰厂(69.5%)、清洗禽类污水(67.9%)和禽肉案板表面拭物(57.1%)。结论湖州市禽类环境中禽流感病毒污染较为严重,且型别多样,存在人感染禽流感病毒的风险,应加强禽类环境中禽流感病毒实时监测。
Objective To understand the avian influenza virus contamination in poultry environment in Huzhou City from 2013 to 2016 and assess the risk of bird flu infection in humans. Methods Samples of poultry drinking water and other environmental samples were collected. Fluorescent RT-PCR was used to detect the influenza A virus nucleic acid samples. Positive samples were further tested for H5, H7 and H9 subtype avian influenza virus nucleic acids. Results A total of 5 867 environmental samples were collected and tested from 2013 to 2016. The total positive rate of influenza A virus was 38.5%. The majority of H7 subtypes were detected (23.6%). The peak positive rate of influenza virus was found in the winter and spring seasons. The positive rates were high in the urban and rural live poultry market (77.8%), poultry slaughtering plant (69.5%), poultry washing wastewater (67.9%) and poultry board surface swab Things (57.1%). Conclusion The avian influenza virus in poultry environment in Huzhou City is more polluted, and its type is diverse. There is a risk of human being infected with avian influenza virus. The real-time monitoring of avian influenza virus should be strengthened in the poultry environment.