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为探讨LiCl动员间充质干细胞(mesenchymal stem cells,MSCs)的效应及动员后细胞的神经分化潜能.用LiCl动员大鼠后,以CFU-F分析其动员效应,从形态学、表型分子的检测等对动员MSCs鉴定.用β-巯基乙醇和神经元原代培养上清液诱导动员后MSCs向神经元分化.结果表明,CFU-F分析显示LiCl可有效动员外周血MSCs,形态学、表型分子等实验证实动员的细胞为MSCs,并可被β-巯基乙醇和神经元原代培养上清液诱导,表达神经元特异标志分子NSE,NF,Nestin和NeuN.全细胞膜片钳记录到诱导后的MSCs有内向钠电流.研究表明,循环MSCs可被LiCl动员,并在一定的环境下向神经元细胞分化,这为神经损伤的修复提示了广阔的前景.
To explore the effect of LiCl mobilizing mesenchymal stem cells (MSCs) and the neural differentiation potential of the cells after mobilization, the mobilization effect was analyzed by CFU-F after mobilization with LiCl, and the effects of morphological and phenotypic molecular Detection and other identification of mobilized MSCs.The primary differentiation of MSCs into neurons was induced by β-mercaptoethanol and neuronal primary culture supernatant.The results showed that CFU-F analysis showed that LiCl can effectively mobilize MSCs in peripheral blood, morphology, Type molecules and other experiments confirmed that the mobilized cells were MSCs and could be induced by β-mercaptoethanol and neuronal primary culture supernatant to express neuron-specific marker molecules NSE, NF, Nestin and NeuN. Whole-cell patch-clamp recording was induced After MSCs have an inward sodium current.Research shows that circulating MSCs can be mobilized by LiCl and differentiate into neuronal cells in a certain environment, which prompts a broad prospect for the repair of nerve injury.