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目的观察胰岛素抵抗高血压大鼠血小板Ｌ－精氨酸／一氧化氮系统的改变。方法自发性高血压大鼠（ＳＨＲ）自第５周至第１０周喂食果糖，制备胰岛素抵抗高血压大鼠（ＩＲ）模型。在此模型上，检测血小板一氧化氮合酶（ＮＯＳ）活性、一氧化氮（ＮＯ）产生量及Ｌ－精氨酸（Ｌ－Ａｒｇ）转运特征，同时观察了血浆Ｌ－Ａｒｇ水平、心钠素（ＡＮＰ）、ＮＯ水平及ｃＧＭＰ水平。结果ＳＨＲ大鼠收缩压（ＳＢＰ）（Ｐ＜０．０１），血浆ＡＮＰ（Ｐ＜０．０１）和ｃＧＭＰ（Ｐ＜０．０５）水平均较ＷＫＹ对照组明显升高，而血糖和血浆胰岛素水平无显著变化（Ｐ＞０．０５）。ＩＲ大鼠的ＳＢＰ（Ｐ＜０．０１），血浆ＡＮＰ（Ｐ＜０．０５）和ｃＧＭＰ（Ｐ＜０．０５）水平较ＳＨＲ进一步升高；且血糖与血浆胰岛素水平升高明显（Ｐ＜０．０１），三组间血浆Ｌ－Ａｒｇ水平并无明显差异（Ｐ＞０．０５）。ＩＲ大鼠血小板ＮＯ产生量、ＮＯＳ活性及血浆ＮＯ水平分别较ＳＨＲ降低２５％（Ｐ＜０．０１）、２４％（Ｐ＜０．０１）和２９％（Ｐ＜０．０１）。Ｌ－Ａｒｇ最大转运速率ＳＨＲ是ＷＫＹ的６０％（Ｐ＜０．０１）、ＩＲ又为ＳＨＲ的８２％（Ｐ＜０．０１），三组间米氏常数（Ｋｍ）的变化无统计学意义（Ｐ＞０?
Objective To observe the changes of platelet L-arginine / nitric oxide system in insulin resistance hypertensive rats. Methods Spontaneous hypertensive rats (SHR) were fed fructose from week 5 to week 10 to prepare insulin resistance hypertensive rats (IR) model. On this model, the activity of nitric oxide synthase (NOS), the production of nitric oxide (NO) and L-arginine (L-Arg) transporter were detected. The levels of plasma L-Arg, (ANP), NO levels and cGMP levels. Results SBP (P <0.01), plasma ANP (P <0.01) and cGMP (P <0.05) levels in SHR rats were significantly higher than those in WKY control group, while the levels of plasma glucose and plasma insulin No significant change in level (P> 0.05). SBP (P <0.01), plasma ANP (P <0.05) and cGMP (P <0.05) levels in IR rats were higher than those in SHR group, and the levels of blood glucose and plasma insulin increased significantly (P < 0.01). There was no significant difference in plasma L-Arg level among the three groups (P> 0.05). Compared with SHR, NO production, NOS activity and plasma NO level in IR rats decreased by 25% (P <0.01), 24% (P <0.01) and 29% (P <0.01), respectively. The maximum translocation rate of L-Arg was 60% (P <0.01) of WKY and 82% of that of SHR (P <0.01). There was no significant difference in Km between the three groups (P> 0?