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pho P/pho Q为沙门菌的双成分调节子 ,pho Q可感受巨噬细胞中一些因子的改变 ,显示组氨酸激酶活性 ,使 pho P氨基端特定的残基磷酸化 ,磷酸化后的 pho P结合于特定的启动子 ,诱导或抑制一系列基因的表达。受 pho P/pho Q激活的 pag C基因的启动子 ppag C能有效地驱动外源基因在鼠伤寒杆菌中的表达 ,激发宿主抗该外源蛋白的抗体。使用 ppag C可克服在减毒沙门菌中因外源基因的组成性表达而带来的低表达和表达质粒不稳定的问题。因此 ,ppag C在以减毒沙门菌为载体的疫苗研制中具有很好的应用前景。
pho P / pho Q is a two-component regulator of Salmonella pho Q can sense some changes in macrophages, showing histidine kinase activity, phosphorylation of specific residues of pho P N-terminal, phosphorylation Pho P binds to specific promoters, inducing or inhibiting the expression of a series of genes. The ppag C promoter of pag C gene activated by pho P / pho Q can effectively drive the expression of the foreign gene in S. typhimurium and stimulate the host antibody against the foreign protein. The use of ppag C overcomes the problem of low expression in the attenuated Salmonella due to the constitutive expression of foreign genes and instability of the expression plasmid. Therefore, ppag C has good application prospects in the development of vaccines with attenuated Salmonella as carrier.