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目的 探讨载脂蛋白CⅢ (apoCⅢ )基因SstⅠ酶切位点多态性与 2型糖尿病合并高甘油三酯血症 (HTG)的关系。方法 应用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)检测 143例 2型糖尿病患者及 12 7例非糖尿病对照者的SstⅠ位点多态性。结果 apoCⅢSstI位点在两组中均以S1等位基因为主。S2 频率在 2型糖尿病组稍高于对照组 (0 .336vs 0 .2 76 ,P >0 .0 5 )。两组中合并高甘油三酯血症 (HTG)者S2 S2 基因型及S2 等位基因频率均高于正常血脂 (NTG)者 (均P <0 .0 5 )。两组中具S2 S2 基因型者apoCⅡ、CⅢ水平较S1S1基因型者明显升高 (P <0 .0 5 ) ,体重指数(BMI)、腰臀比 (WHR)、甘油三酯 (TG)、总胆固醇 (TC)、载脂蛋白B(apoB)、载脂蛋白E(apoE)水平较S1S1和S1S2 基因型者有增高趋势 ,HDL C和胰岛素敏感指数 (ISI)有下降趋势 (P >0 .0 5 )。结论ApoCⅢSstⅠ位点S2 等位基因可能是造成 2型糖尿病人群发生HTG的遗传因素之一。
Objective To investigate the relationship between Sst Ⅰ restriction site polymorphism of apolipoprotein C (apoC Ⅲ) gene and hypertriglyceridaemia (HTG) in type 2 diabetes mellitus (T2DM). Methods Polymorphisms of SstⅠ in 143 patients with type 2 diabetes mellitus and 12 7 non-diabetic controls were detected by polymerase chain reaction restriction fragment length polymorphism (PCR RFLP). Results The apoCⅢSstI locus was predominantly the S1 allele in both groups. S2 frequency in type 2 diabetes group slightly higher than the control group (0.336 vs 0.276, P> 0.05). S2 S2 genotype and S2 allele frequency in patients with hypertriglyceridemia (HTG) were higher than those with normal blood lipid (NTG) in both groups (P <0.05). The levels of apoCⅡ and CⅢ in patients with S2 S2 genotype were significantly higher than those in S1S1 genotype (P <0. 05), body mass index (BMI), WHR, triglyceride TC, apoB and apoE increased compared with S1S1 and S1S2 genotypes, while HDL C and ISI showed a decreasing trend (P> 0.05). 0 5). Conclusion The S2 allele of ApoCⅢSstⅠis probably one of the genetic determinants of HTG in type 2 diabetic patients.