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背景与目的:肿瘤疫苗作为一种肿瘤特异性主动免疫治疗的手段,在其生物学治疗中起着重要作用。本研究探讨免疫佐剂胞嘧啶鸟嘌呤二核苷酸-脱氧寡核苷酸(cytidine-phosphatte-guanonine oligodeoxynucleotides,CpG ODN)1826在白血病动物模型中的抗瘤作用和毒副作用。方法:建立急性淋巴细胞白血病动物模型,在不同时间用灭活的L1210细胞和免疫佐剂皮下注射给DBA/2小鼠,观察小鼠一般状况、成瘤率、而且瘤块生长情况;通过病理切片了解瘤块中心及周围浸润细胞情况。结果:与对照组比,灭活细胞加GpG ODN1826瘤苗可以降低小鼠成瘤率,而且成瘤小鼠瘤块有消退现象,瘤周单个核细胞浸润明显,瘤块内肿瘤细胞坏死明显;但生存期无明显延长。结论:应用GpG ODN1826瘤苗可以加强白血病小鼠的抗瘤作用,但非荷瘤小鼠有死亡现象,需对GpG ODN1826瘤苗进行改进或筛选其他适合临床应用的免疫佐剂。
Background and Objective: Tumor vaccines, as a means of tumor-specific active immunotherapy, play an important role in their biological therapy. This study was aimed to investigate the anti-tumor effects and side effects of the adjuvant cytidine-phosphatte-guanonine oligodeoxynucleotides (1826) in animal models of leukemia. Methods: Animal models of acute lymphoblastic leukemia were established. DBA / 2 mice were instilled subcutaneously with inactivated L1210 cells and immune adjuvant at different times to observe the general condition, tumor formation rate and tumor growth of mice. Slice to understand the tumor center and surrounding infiltrating cells. Results: Compared with the control group, the inactivated cells plus GpG ODN1826 could reduce the tumor formation rate in mice, and the tumors in tumor - forming mice subsided. The infiltration of peripheral mononuclear cells was obvious and the necrosis of tumor cells in tumor mass was obvious. But no significant extension of survival. Conclusion: GpG ODN1826 can enhance the anti-tumor effects of leukemia mice, but the death of non-tumor-bearing mice need to be improved or screening GpG ODN1826 other adjuvants suitable for clinical application.