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目的观察DSCR1基因在喉咽癌组织和癌旁组织中的表达及与微血管密度(MVD)之间的关系,探讨DSCR1基因对喉咽癌的血管新生的生物学行为的影响。方法收集喉咽鳞状细胞癌患者94例,其中78例取癌旁组织,通过免疫组织化学P-V9000法,使用兔抗DSCR1抗体DCT3,检测DSCR1基因在喉咽鳞状细胞癌与正常癌旁组织中的表达,通过CD34抗体染色标记血管内皮细胞,计算MVD,将DSCR1基因表达与MVD之间做相关性分析。结果肿瘤组织中DSCR1基因表达阳性率94.9%,癌旁组织表达阳性率35.9%,差异有统计学意义(t=23.69,P<0.001);喉咽癌组织平均MVD(17.57±10.61)条/400倍视野,癌旁正常组织(12.61±7.67)条/400倍视野(P<0.05)。在喉咽癌肿瘤组织中随着DSCR1基因的表达增强,MVD也逐渐升高(P<0.001),两者呈正相关性(r=0.698)。结论 DSCR1基因在喉咽鳞状细胞癌中表达增加,并与其血管生成的生物学行为密切相关,可以作为一个新的基因治疗靶向。
Objective To investigate the expression of DSCR1 in hypopharyngeal carcinoma tissues and adjacent tissues and its relationship with microvessel density (MVD). To investigate the effect of DSCR1 gene on the biological behavior of angiogenesis in hypopharyngeal carcinoma. Methods Ninety-four patients with squamous cell carcinoma of the larynx pharynx were collected, including 78 cases of paracancerous tissues. Immunohistochemistry P-V9000 method and rabbit anti-DSCR1 antibody DCT3 were used to detect the expression of DSCR1 in squamous cell carcinoma of the larynx pharynx and adjacent normal tissues Tissue samples were collected and labeled with CD34 antibody to label vascular endothelial cells. The MVD was calculated and the correlation between DSCR1 gene expression and MVD was analyzed. Results The positive rate of DSCR1 gene was 94.9% in tumor tissues and 35.9% in adjacent non-cancerous tissues (t = 23.69, P <0.001). The mean MVD (17.57 ± 10.61) / 400 (12.61 ± 7.67) / 400 times (P <0.05). MVD also increased gradually with the increase of DSCR1 gene expression in the tumor tissues of hypopharyngeal carcinoma (P <0.001), which showed a positive correlation (r = 0.698). Conclusion The expression of DSCR1 gene is increased in squamous cell carcinoma of the hips and pharynx, and is closely related to the biological behavior of angiogenesis. DSCR1 gene may serve as a new gene therapy target.