采用高效液相色谱法（ＨＰＬＣ）对复方康希力胶囊中氨苄青霉素和邻氯青霉素进行测定。以醋酸盐缓冲液（ｐＨ＝４．５）－甲醇（５５：４５）为流动相，流速为１．０ｍＬ／ｍｉｎ；ＹＷＧ－Ｃ＿（１８）（１０μｍ）为固定相；乙酰苯胺为内标；检测波长为２５４ｎｍ。氨苄青霉素、内标物和邻氯青霉素的保留时间分别为３，０、５．５和９．０ｍｉｎ。氨苄青霉素和邻氯青霉素的回收率（ｎ＝５）分别为１００．３％（ＲＳＤ＝０．３７％）和１００．９％（ＲＳＤ＝０．４３％）。两种组分的最低检出浓度为５μｇ／ｍＬ。 The contents of ampicillin and cloxacillin in compound Kangxi Li capsules were determined by high performance liquid chromatography (HPLC). The mobile phase consisted of acetate buffer (pH = 4.5) -methanol (55:45) with the flow rate of 1.0 mL / min and the YWG-C 18 (10 μm) ; Detection wavelength of 254nm. The retention times of ampicillin, internal standard and o -chloromycin were 3, 0, 5.5 and 9.0 min, respectively. The recoveries (n = 5) of ampicillin and o -chloromycin were 100.3% (RSD = 0.37%) and 100.9% (RSD = 0.43%), respectively. The minimum detectable concentration of both components was 5 μg / mL.