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目的探讨滇重楼不同居群间、同一居群不同植株间甾体皂苷活性成分在根茎、茎、叶中的代谢规律。方法采用超高效液相色谱法(UPLC)分别测定滇重楼不同居群间、同一居群不同植株间根茎、茎、叶中甾体皂苷活性成分的含量,并建立其UPLC图谱,对滇重楼不同居群间、同一居群不同植株间甾体皂苷活性成分在根茎、茎、叶的代谢规律进行研究。结果滇重楼不同居群间根茎、茎、叶中次生代谢产物重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ及总皂苷含量(P<0.01)具有极显著差异,UPLC图谱中色谱峰个数为叶>根茎>茎;滇重楼同一居群不同植株间根茎、茎、叶中次生代谢产物重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ及总皂苷含量(P<0.05)差异较小,UPLC图谱中色谱峰1、2、4、5在根茎、茎、叶均存在,但叶比根茎、茎分别还多11、16个色谱峰。结论滇重楼不同居群间、同一居群不同植株的茎中主要活性成分含量较低、种类较少;滇重楼甾体皂苷活性成分在同一居群不同植株间根茎、茎、叶中的代谢具有较稳定的居群遗传能力,在不同居群间的代谢存在较大的差异;滇重楼茎、叶是否可以作为其根茎的替代品入药,仍需进一步做药理、毒理及临床实验。
Objective To investigate the metabolism of steroidal saponin active ingredients in different rhizomes, stems and leaves among different populations of the same species in the same population. Methods The contents of active constituents in the rhizomes, stems and leaves of rhizomes, stems and leaves of different populations of Diannonglou were detected by UPLC. The UPLC patterns were established. The metabolic regularities of the active ingredients of steroidal saponins in rhizomes, stems and leaves among different populations of the same building and between different plants in the same population were studied. Results The content of secondary metabolites of resplendent compounds Ⅰ, Ⅱ, Ⅵ, Ⅶ and total saponins (P <0.01) in rhizomes, stems and leaves of different populations of Diannonglou were significantly different. The number of peaks in UPLC was Leaves> rhizomes> stems. There was no significant difference in the content of secondary metabolites of Rhizoma Corydalis Ⅰ, Ⅱ, Ⅵ, Ⅶ and total saponins (P <0.05) between the rhizomes, stems and leaves of different plants of the same population. The chromatographic peaks 1, 2, 4, and 5 were present in rhizomes, stems and leaves, but there were 11,16 more peaks in rhizomes and stems. Conclusion The main active components in the stems of different plants of the same population were lower and less in different populations of D. reticulata. The active constituents of steroidal saponins from the rhizomes, stems and leaves of different plants in the same population Metabolism has a more stable genetic ability of the population, there is a big difference in the metabolism among different populations. Whether the stems and leaves of Dianhonglou can be used as a substitute for their rhizomes still need further pharmacological, toxicological and clinical trials .