重庆市公共场所水环境嗜肺军团菌污染研究

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目的了解重庆市公共场所水系统嗜肺军团菌污染现状,监测公共场所水系统清洗消毒效果,为卫生监督管理提供依据。方法依照卫生部《公共场所集中空调通风系统卫生规范》(2006年)附录A方法(2013-04-01前)和《公共场所集中空调通风系统卫生规范》WS 394-2012附录B方法(2013-04-01实施),采集本市多家公共场所冷却水、冷凝水、冷冻水、温泉水、淋浴水、游泳池水、生活用水等共399件水样品,进行嗜肺军团菌检测以及菌型分布检测。结果在399件样品中检出44件阳性,阳性率为11.0%;空调系统水嗜肺军团菌阳性检出率逐渐下降(29.0%/15.6%/9.0%),差异有统计学意义(χ~2=8.53,P<0.05);空调系统水嗜肺军团菌检出率高于生活水嗜肺军团菌检出率(13.9%/7.1%),差异有统计学意义(χ~2=4.608,P<0.05);检出44株嗜肺军团菌,其中LP1型有32株(72.7%);LP2-14型有12株(27.3%)。结论本市公共场所水系统中存在嗜肺军团菌污染,应加强公共场所水系统的清洗消毒,严格监管。 Objective To understand the current status of Legionella pneumophila contamination in water systems in public places in Chongqing and to monitor the cleaning and disinfection effects of water systems in public places so as to provide the basis for health supervision and management. Methods According to the method of appendix A of Ministry of Health, “Hygienic Standard for Central Air Conditioning Ventilation System in Public Places” (2006) (before 2013-04-01) and Hygienic Practice of Central Ventilation and Ventilation System in Public Places WS 394-2012 Appendix B Method (2013- 04-01 implementation), collecting a total of 399 water samples from a number of public places in the city, including cooling water, condensate, chilled water, hot spring water, shower water, swimming pool water and domestic water, to detect Legionella pneumophila and the distribution of bacteria Detection. Results The positive rate was 11.0% in 399 samples. The positive detection rate of Legionella pneumophila in air-conditioning system was gradually decreased (29.0% / 15.6% / 9.0%), the difference was statistically significant (χ ~ 2 = 8.53, P <0.05). The detection rate of Legionella pneumophila in air-conditioning system was higher than that of Legionella pneumophila (13.9% / 7.1%) in air-conditioning system with a significant difference (χ ~ Forty-four strains of Legionella pneumophila were detected, of which 32 (72.7%) were LP1 and 12 (27.3%) were LP2-14. Conclusion There is Legionella pneumophila pollution in the water system of public places in this Municipality, the cleaning and disinfection of water system in public places should be strengthened and the supervision should be strictly regulated.
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