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目的:探讨检测非小细胞肺癌(NSCLC)患者外周血Lunx mRNA表达对于诊断肿瘤微转移的临床价值。方法:采用荧光定量RT-PCR技术分别对98例NSCLC患者和20例正常对照组外周血Lunx mRNA的表达进行检测分析。结果:NSCLC组患者外周血Lunx mRNA的阳性率为74.5%,显著高于对照组的15%(x~2=25.642,P<0.05);Ⅲ期NSCLC患者的阳性率显著高于Ⅰ期和Ⅱ期患者的阳性率(x~2=21.014,P<0.05);淋巴结转移阳性和阴性患者的外周血中CK19mRNA阳性率分别为93.2%和46.2%,差异有统计学意义(x~2=27.372,P<0.05);不同细胞分化程度与NSCLC患者外周血微转移阳性率有密切关系(x~2=30.004,P<0.05);NSCLC患者外周血微转移与年龄、性别、吸烟史、原发肿瘤部位无密切关系(P>0.05)。结论:Lunx mRNA的高表达可能与非小细胞肺癌的微转移有关,可作为检测NSCLC患者微转移的分子标志物,其有助于早期诊断肺癌转移,指导临床分期和治疗。
Objective: To investigate the clinical value of detecting the expression of Lunx mRNA in the peripheral blood of patients with non-small cell lung cancer (NSCLC) for the diagnosis of micrometastasis. Methods: The expression of Lunx mRNA in peripheral blood of 98 patients with NSCLC and 20 normal controls were detected by fluorescence quantitative RT-PCR. Results: The positive rate of Lunx mRNA in peripheral blood of patients with NSCLC was 74.5%, which was significantly higher than that of control group (x ~ 2 = 25.642, P <0.05). The positive rate of patients with stage Ⅲ NSCLC was significantly higher than that of patients with stage Ⅰ and Ⅱ (X ~ 2 = 21.014, P <0.05). The positive rates of CK19 mRNA in peripheral blood of positive and negative patients with lymph node metastasis were 93.2% and 46.2%, respectively, with significant difference (x 2 = 27.372, P <0.05). Differentiation of different cells was closely related to the positive rate of micrometastasis in peripheral blood of patients with NSCLC (x ~ 2 = 30.004, P <0.05); micrometastasis in peripheral blood of patients with NSCLC was significantly related to age, sex, smoking history, There is no relationship between the site (P> 0.05). Conclusion: The high expression of Lunx mRNA may be related to the micrometastasis of non-small cell lung cancer. It may be used as a molecular marker for the detection of micrometastasis in NSCLC patients. It is helpful for the early diagnosis of lung cancer metastasis and clinical staging and treatment.