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[目的]观察芪薏益肝煎对H22荷瘤小鼠的抑瘤作用,并探讨其作用机制。[方法]小鼠右腋下皮下种瘤制作实体瘤模型,将30只小鼠分为3组:对照组、中药组和5-氟脲嘧啶(5-Fu)组,各10只,用药15 d后取瘤称重计算抑瘤率;采用免疫组化技术检测存活素(Survivin)和细胞周期素(Cyclins)D1蛋白的表达。[结果]中药组对小鼠肝癌细胞的生长抑制率为37.28%,5-Fu组为50.48%。Survivin及Cyclin D1在3组中均呈阳性表达,中药组Sur-vivin和Cyclin D1表达减弱,与对照组比较差异有统计学意义(P<0.05,<0.01)。[结论]芪薏益肝煎对肝癌细胞的增殖有明显的抑制作用,其作用机制与Survivin及Cyclin D1表达有关。
[Objective] To observe the anti-tumor effect of Qiyi Yigangan decoction on H22 tumor-bearing mice and to explore its mechanism. [Method] The right subaxillary subcutaneous tumor of mice was used to make solid tumor model. Thirty mice were divided into three groups: control group, traditional Chinese medicine group and 5-Fu group, each with 10 The tumors were weighed and the tumor inhibition rate was calculated. The expression of Survivin and Cyclin D1 was detected by immunohistochemistry. [Result] The growth inhibition rate of Chinese medicine group on mouse hepatoma cells was 37.28% and that of 5-Fu group was 50.48%. Survivin and Cyclin D1 were all expressed in all three groups. The expressions of Survivin and Cyclin D1 in the Chinese herbs group were significantly lower than those in the control group (P <0.05, <0.01). [Conclusion] Qiyi Yigangan decoction significantly inhibited the proliferation of hepatocellular carcinoma cells, and its mechanism of action was related to the expression of Survivin and Cyclin D1.