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目的:探讨细胞凋亡抑制基因B-淋巴瘤细胞-2(B-cell lymphoma/leuke mia-2,bcl-2)和血管内皮生长因子(Vascular endothelial growth factor,VEGF)在肾母细胞瘤中的表达及其相互关系。方法:用免疫组织化学和原位杂交方法研究bcl-2和VEGF蛋白及mRNA在17例肾母细胞瘤和7例瘤旁正常肾组织的表达。结果:bcl-2蛋白在肾母细胞瘤组织中的表达(82.4%)高于瘤旁正常肾组织(28.5%);VEGF蛋白在肾母细胞瘤组织中的表达(58.8%)高于瘤旁正常肾组织(14.3%);bcl-2 mRNA和VEGF mRNA在肾母细胞瘤的表达率分别为88.2%和82.4%;bcl-2和VEGF的表达之间存在正相关关系(rs=0.633,P<0.01)。结论:bcl-2和VEGF在小儿肾母细胞瘤发生发展中起重要作用。
Objective: To investigate the expression of apoptosis-inducing gene B-cell lymphoma / leukemia-2 (Bcl-2) and vascular endothelial growth factor (VEGF) in nephroblastoma Expressions and their relationships. Methods: The expressions of bcl-2 and VEGF protein and mRNA in 17 cases of nephroblastoma and 7 cases of normal neoplasm were studied by immunohistochemistry and in situ hybridization. Results: The expression of bcl-2 protein in nephroblastoma was higher than that in normal neoplasm (28.5%). The expression of VEGF protein in nephroblastoma was higher than that in neoplastic The expression of bcl-2 mRNA and VEGF mRNA in nephroblastoma was 88.2% and 82.4%, respectively. There was a positive correlation between the expression of bcl-2 and VEGF (rs = 0.633, P <0.01). Conclusion: bcl-2 and VEGF play an important role in the development of pediatric nephroblastoma.