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目的:观察比较黄芪注射液和经典洋地黄类强心药去乙酰毛花苷注射液对戊巴比妥钠和心得安所致不同大鼠急性心衰模型的强心作用。方法:48只健康Wistar大鼠随机分为戊巴比妥钠模型组、戊巴比妥钠模型去乙酰毛花苷组、戊巴比妥钠模型黄芪注射液组、心得安模型组、心得安模型去乙酰毛花苷组和心得安模型黄芪注射液组,每组8只。分别于静脉输入戊巴比妥钠或心得安制作大鼠急性心衰模型,戊巴比妥钠模型去乙酰毛花苷组和心得安模型去乙酰毛花苷组在造模成功后均给予去乙酰毛花苷注射液(0.1 mg.kg-1),戊巴比妥钠模型黄芪注射液组和心得安模型黄芪注射液组在造模成功后均给予黄芪注射液(4 ml.kg-1),检测各组大鼠在造模成功并稳定20min后,静脉注射去乙酰毛花苷注射液或黄芪注射液5min、15min、30min、60min和120min各时间点LVSP、+dp/dtmax、HR等左室收缩功能的变化。结果:2种造模方法均能使大鼠LVSP、+dp/dtmax、HR显著降低,且在2h内保持在一个较低水平,显示大鼠急性心衰模型制作成功而稳定;静脉注射黄芪注射液使2种模型大鼠的LVSP、+dp/dtmax均显著升高,心得安模型大鼠黄芪注射液起效更为迅速,于药后5min即出现了明显的正性肌力作用(P<0.05);戊巴比妥钠模型大鼠药后15min才出现心肌收缩力的明显增强(P<0.05)。结论:与戊巴比妥钠所致急性心衰模型相比,心得安诱发的急性心衰模型更适合于黄芪注射液治疗急性心衰的药理药效学研究。
OBJECTIVE: To observe and compare the cardioprotective effects of Astragalus injection and classic digitalis cardibosides on acetylcholine glycosides in pentobarbital sodium and propranolol-induced acute heart failure model in rats. Methods: Forty-eight healthy Wistar rats were randomly divided into sodium pentobarbital model group, sodium pentobarbital sodium model group, sodium pentobarbital model astragalus injection group, propranolol model group, The model group was treated with acetylglucoside and astragalus injection group, 8 in each group. Respectively, intravenous sodium pentobarbital or propranolol acute rat model of acute myocardial infarction, sodium pentobarbital sodium model group and propranolol glycosides desacetylation model were given after the success of modeling Astragalus injection (4 ml · kg-1) was given after the successful model-making by acetylation of strychnine injection (0.1 mg.kg-1), Astragalus membranaceus model of pentobarbital sodium model group and astragalus injection of propranolol model group ), LVSP, + dp / dtmax, HR, etc. were measured at different time points after 5 min, 15 min, 30 min, 60 min and 120 min after intravenous injection of stilbene glycoside or Astragalus injection. Left ventricular systolic function changes. Results: The LVSP, + dp / dtmax and HR of rats were both significantly reduced and maintained at a lower level within 2 hours. The results showed that the model of acute heart failure was successfully established and stabilized in rats. Intravenous injection of Astragalus injection Liquid made the LVSP and + dp / dtmax of two kinds of model rats increased significantly. Astragalus injection of Ann Model rats started to exert more rapid effect, and obvious inotropic effect appeared 5 minutes after administration (P < 0.05). Myocardial contractility increased significantly after 15 min in pentobarbital sodium model rats (P <0.05). CONCLUSIONS: Compared with sodium pentobarbital-induced acute heart failure model, propranolol-induced acute heart failure model is more suitable for the pharmacodynamic study of astragalus injection in the treatment of acute heart failure.