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[目的]研究外源生长调节剂处理对梨杂交后代的花芽分化、成花相关基因表达及营养物质积累的影响。[方法]以5年生的‘满天红’ב砀山酥梨’杂交后代中尚未开花的实生个体为材料,在花芽分化期进行6-BA和乙烯利(Eth)喷施处理(100 mg·kg-16-BA,1 000 mg·kg-1Eth,100 mg·kg-16-BA+1 000 mg·kg-1Eth),以清水为对照。[结果]不同生长调节剂处理均能促进杂交后代实生苗成花,成花数量显著增加,其中6-BA和乙烯利混合液处理形成的花芽个数最多。各处理均促进了花芽分化期间成花相关基因FLOWERING LOCUS T(FT)和LEAFY(LFY)的表达,抑制了TERMINAL FLOWER 1(TFL1)基因的表达;提高了花芽内玉米素(ZT)和ABA的含量,降低了GA3和IAA的含量,提高了花芽的淀粉含量和C/N比值。[结论]综合分析表明,基因表达、内源激素和营养物质之间相互影响,共同调控杂交后代的成花。
[Objective] The research aimed to study the effects of exogenous growth regulator on flower bud differentiation, flower-related gene expression and nutrient accumulation in hybrids of pear. [Method] With 6-BA and Eth (Eth) spray treatment (5 mg · d -1) as the inflorescences of 5-year-old hybrids of ’Mantianhong’ × ’Dangshansu pear’ kg-16-BA, 1000 mg · kg-1Eth, 100 mg · kg-16-BA + 1000 mg · kg-1Eth). [Result] Different growth regulators could promote the seedling formation of hybrid progenies and the flower number significantly increased. The number of flower buds formed by 6-BA and ethephon mixture was the highest. All the treatments promoted the expression of FLOWERING LOCUS T (FT) and LEAFY (LFY) during flower bud differentiation and inhibited the expression of TERMINAL FLOWER 1 (TFL1) gene and increased the expression of Zeatin (ZT) and ABA Content, reduced GA3 and IAA content, increased flower bud starch content and C / N ratio. [Conclusion] The comprehensive analysis showed that the interaction between gene expression, endogenous hormones and nutrients could regulate the flowering of hybrid progeny.