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目的:探讨线粒体呼吸链膜电位与海洛因海绵状白质脑病的关系。方法:运用荧光探针JC-1对外周血细胞线粒体膜电位进行标记,再通过流式细胞仪检测海洛因海绵状白质脑病患者及正常组膜电位改变情况。结果:海洛因白质脑病组与正常组线粒体膜电位测定结果分别为0.24±0.07和0.18±0.05,两组间具有显著差异(P<0.01)。结论:急性期海洛因海绵状白质脑病患者线粒体膜电位活性较正常组明显下降,它是线粒体调亡信号,推测本病可能的发病机制是,烫吸过程中产生的毒物使海洛因海绵状白质脑病患者机体内细胞的线粒体功能发生了障碍,导致了乳酸的堆积,从而引起对缺氧敏感的颅内组织出现广泛的白质继发脱髓鞘。
Objective: To investigate the relationship between the mitochondrial respiratory chain membrane potential and herpes cass. Methods: Peripheral blood mitochondrial membrane potential was labeled with fluorescent probe JC-1, and then the changes of membrane potential in heroin causative patients and normal group were detected by flow cytometry. Results: The results of mitochondrial membrane potential measurements of heroin encephalopathy group and normal group were 0.24 ± 0.07 and 0.18 ± 0.05 respectively, with significant difference between the two groups (P <0.01). Conclusion: The mitochondrial membrane potential of patients with herpes spontaneous leuco encephalopathy in the acute phase was significantly lower than that in the normal group. It is a signal of mitochondrial apoptosis. It is speculated that the pathogenesis of this disease is that the toxic substances in the process of hot sweat make the patients with spongiform encephalopathy Mitochondrial dysfunction of cells in the body, leading to the accumulation of lactic acid, causing hypoxia-sensitive intracranial tissue appeared extensive white matter secondary demyelination.