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本工作选用健康雄性wistar大鼠,以放射免疫测定法(RIA),观察了在海拔2260m高原环境中长期生存的大鼠;下丘脑(Hypothalamus),中脑(Mesencephalon)、海马(Hippocampus)β—内啡肽免疫活性物质(β—EPLI)含量的变化。结果表明,海马、中脑β—EPLI含量与平原者对照组相比,下降非常显著(P<0.01);下丘脑与平原者对照组相比,下降亦有显著性意义(P<0.05)。提示,在高原地区,大鼠脑内β—EPLI可能参与其对低氧刺激的长期适应调节过程。
In this study, healthy male Wistar rats were selected and radioimmunoassay (RIA) was used to observe the long-term survival of rats at altitude of 2260m. Hypothalamus, Mesencephalon, Hippocampus β- Changes in the content of endorphin immunoreactive substance (β-EPLI). The results showed that the content of β-EPLI in the hippocampus and midbrain decreased significantly compared with the control group (P <0.01). The hypothalamus and plain control group also showed a significant decrease (P <0.05). These results suggest that β-EPLI may be involved in the regulation of long-term adaptation to hypoxia in rats in the plateau.