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目的 探讨血管紧张素转移酶 (ACE)基因插入 /缺失多态性与儿童哮喘易感性的关系。方法 应用聚合酶链反应方法检测 5 2例哮喘患儿 ,5 4例肺炎患儿及 40例正常儿童的ACE基因型。用呼气峰速仪测定 2 3例哮喘患儿的最大呼气流量作为评价肺功能的指标。结果 三组儿童ACE基因型 (II型、ID型、DD型 )频率的分布差异有显著意义 (P <0 .0 5 )。哮喘组DD基因型频率为 34.6 % ,与正常组 ( 12 .5 % )和肺炎组 ( 14.8% )比较 ,差异有显著意义 (P均 <0 .0 5 )。哮喘组和正常组比较 ,DD型与ID型、DD型与II型的优势比OR分别为 5 .0 4和 3.15 ( 95 %可信区间 )。不同ACE基因型间比较哮喘患儿最大呼气流量占预计值的百分比 ,差异无显著意义 (P >0 .0 5 )。结论 ACE基因DD基因型与哮喘的易感性有关 ,可能是儿童哮喘的危险因素。
Objective To investigate the relationship between angiotensin transferase (ACE) gene insertion / deletion polymorphism and childhood asthma susceptibility. Methods ACE genotypes were detected in 52 children with asthma, 54 children with pneumonia and 40 normal children by polymerase chain reaction. The maximum expiratory flow of 23 asthmatic children was measured by the breath peak velocity meter as an index to evaluate the pulmonary function. Results The frequencies of ACE genotypes (type II, type ID and type DD) in three groups were significantly different (P <0.05). The frequency of DD genotype in asthma group was 34.6%, which was significantly different from that in normal group (12.5%) and pneumonia group (14.8%) (all P <0.05). The odds ratios (OR) of DD type to ID type, DD type and II type were respectively 6.04 and 3.15 (95% confidence interval) in asthma group and normal group. The percentage of maximum expiratory flow in children with different ACE genotypes compared with the predicted value had no significant difference (P> 0.05). Conclusion The DD genotype of ACE gene is associated with asthma susceptibility and may be a risk factor for childhood asthma.