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目的:探讨粘质沙雷菌苗对支气管哮喘的免疫调节作用。方法:雄性大鼠40只,随机分为正常对照组(A组)、免疫组(B组)、哮喘模型组(C组)、常规哮喘模型组(D组),每组10只。A组仅注射PBS液,B、C、D组在皮内注射粘质沙雷菌苗。8周后,B组用PBS液代替,C组使用BCG免疫、D组未使用BCG免疫建立哮喘模型。对各组支气管肺泡灌洗液(BALF)中炎性细胞及细胞因子进行检测,并行方差分析。结果:B、C、D组在BALF中淋巴细胞多于正常对照组。B、C组较D组Th2型IL-4、IL-5分泌减少,细胞因子向Th1型明显偏移。肺组织HE染色后,C组见支气管狭窄较B组减轻。结论:粘质沙雷菌苗可明显促进Th1型IFN-γ的分泌,具有预防及治疗支气管哮喘的前景。
Objective: To investigate the immunomodulatory effect of mucilaginous vaccine on bronchial asthma. Methods: Forty male rats were randomly divided into normal control group (group A), immunized group (group B), asthma model group (group C) and conventional asthma model group (group D) with 10 rats in each group. Group A was injected with PBS solution only, while group C was injected intradermally with Serratia marcescens vaccine. After 8 weeks, group B was replaced by PBS solution, group C was immunized with BCG, and group D was not immunized with BCG to establish an asthma model. The inflammatory cells and cytokines in bronchoalveolar lavage fluid (BALF) of each group were detected and analyzed by ANOVA. Results: There were more lymphocytes in BALF than those in normal control in groups B, C and D. The secretion of Th2-type IL-4 and IL-5 in group B and C decreased compared with group D, and the cytokines shifted to Th1. Lung tissue HE staining, C group, see the bronchus stenosis than the B group reduced. Conclusions: Serratia marcescens vaccine can significantly promote the secretion of Th1-type IFN-γ, with the prospect of prevention and treatment of bronchial asthma.