在筛选和鉴定水稻T-DNA(含Basta抗性基因Bar)插入纯合体的过程中,观察到1个白化苗的突变体.对该突变体进行遗传分析表明,分离群体出现绿苗和白化苗2种类型,其分离比率为3∶1,符合1对基因的显性遗传.Basta抗性检测、PCR分子检测及Southern杂交证实,该突变体是由单一T-DNA插入所引起的,白化苗性状与T-DNA共分离.该突变材料可用于插入座位的基因克隆. A mutant of albinism was observed in the screening and identification of homozygotes for insertion of T-DNA in rice (including Basta resistance gene Bar). Genetic analysis of the mutant showed that green and blue algae emerged 2 isolates with a ratio of 3: 1, which is in line with the dominant inheritance of one pair of genes.The detection of Basta resistance, PCR and Southern hybridization confirmed that the mutant was caused by a single T-DNA insertion, The trait was co-segregated with T-DNA and the mutated material was used for gene cloning inserted into the seat.