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目的探讨乙型肝炎病毒前S1抗原(Pre S1-Ag)在乙型肝炎病毒(HBV)感染和复制中的应用价值。方法采用酶联免疫技术(ELISA)对4 068例血清进行Pre S1-Ag和HBV血清标志物检测,并用荧光定量聚合酶链反应(FQ-PCR)检测HBV-DNA。结果 Pre S1-Ag的阳性检出率在HBSAg(+)组与HBSAg(-)组中,差别有统计学意义(P<0.05),Pre S1-Ag在HBSAg(+)组中有较高的检出率,检出率为68.10%,在HBSAg(-)组中,Pre S1-Ag有4例阳性例数。在HBe Ag(+)组和HBe Ag(-)组中Pre S1-Ag、HBV-DNA的阳性检出率分别为85.20%、88.20%和31.40%、33.40%,差异有统计学意义(P<0.05)。结论 Pre S1-Ag是判断HBV复制传染性的指标,也可作为乙肝病毒早期感染的诊断指标之一。Pre S1-Ag和乙肝二对半应同步检测,以提高HBV感染的检出率,防止漏诊。
Objective To investigate the value of Pre S1-Ag in hepatitis B virus (HBV) infection and replication. Methods PreS-Ag and HBV serum markers were detected by enzyme-linked immunosorbent assay (ELISA) on 4068 serum samples and HBV-DNA was detected by FQ-PCR. Results The positive detection rate of Pre S1-Ag in HBSAg (+) group and HBSAg (-) group was significantly different (P <0.05). Pre S1-Ag in HBSAg (+) group was higher The detection rate was 68.10%. In the HBSAg (-) group, there were 4 positive cases for Pre S1-Ag. The positive rates of Pre S1-Ag and HBV-DNA in the HBeAg (+) and HBeAg (-) groups were 85.20%, 88.20% and 31.40%, 33.40%, respectively, with significant difference (P < 0.05). Conclusion Pre S1-Ag is an indicator of HBV replication and can be used as one of the diagnostic indicators of early infection of hepatitis B virus. Pre S1-Ag and hepatitis B should be two pairs of semi-synchronous testing to improve the detection rate of HBV infection, to prevent missed diagnosis.