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基于芘(Py)单体荧光光谱结构对微观环境变化的敏感性,以及介质粘度及Py分子间距对Py激基缔合物形成的影响,以戊二醛交联壳聚糖(CS)水凝胶体系为例研究了Py荧光探针法监测水凝胶形成过程及其溶胀性能的可行性.结果表明,Py荧光光谱精细结构的变化(以I_3/I_1为参量)或Py激基缔合物荧光强度与单体荧光强度之比(I_E/I_M)的变化与CS水凝胶的形成及溶胀程度有很好的对应关系.此外,CS凝胶网状结构中包埋的聚N-异丙基丙烯酸胺(PNIPAM)的构象变化也可由探针光谱变化反映出来.利用这种荧光探针方法有助于从分子水平上探知凝胶形成过程的微观本质.此外,这种方法也可作为光纤传导监测凝胶形成过程和溶胀的基础.
Based on the sensitivity of pyrene (Py) monomer to the change of microscopic environment and the influence of medium viscosity and Py spacing on Py excimer formation, glutaraldehyde cross-linked chitosan (CS) hydrogel Gel system as an example, the feasibility of using Py fluorescence probe to monitor hydrogel formation process and its swelling behavior was studied. The results showed that the change of fine structure of Py fluorescence spectrum (based on I_3 / I_1) or Py excimer Fluorescence intensity and monomer fluorescence intensity (I_E / I_M) changes with the CS hydrogel formation and swelling degree has a good correspondence between.In addition, CS gel network embedded in poly N-isopropyl The conformational change of PNIPAM can also be reflected by the change of the spectrum of the probe.Using this fluorescent probe method can help to discover the microscopic nature of the gel formation process at the molecular level.In addition, this method can also be used as an optical fiber Conduction monitors the gel formation process and the basis of swelling.