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采用分子杂交的方法观察了肾病综合征大鼠白蛋白ｍＲＮＡ的表达水平。旨在从分子水平上探讨黄芪，当归的治疗机理。各组大鼠肝脏白蛋白ｍＲＮＡ的Ｎｏｒｔｈｅｒｎ杂交分析表明，白蛋白的ｍＲＮＡ条带位于２．１ｋｂ处，吸光度（Ａ）肾病组（１．７０±０．１２）高于对照组（１．２５±０．２４）而治疗组（２．５６±０．２４）又高于肾病组（Ｐ＜０．０１）。在富蛋白饮食的基础上，黄芪，当归可使肾病综合征大鼠肝脏白蛋白ｍＲＮＡ表达上调，从而使得大鼠在每日尿中丢失蛋白量不减的情况下，血浆白蛋白保持在近于正常的范围（３７±１．０ｇ／Ｌ）。此结果提示通过增加肾病鼠肝白蛋白ｍＲＮＡ的水平，使肝白蛋白的合成增加，提高了血浆白蛋白的水平，改善蛋白质代谢紊乱。
The expression of albumin mRNA in rats with nephrotic syndrome was observed by molecular hybridization. The aim is to explore the therapeutic mechanism of Radix Astragali and Angelica on the molecular level. Northern blot analysis of liver albumin mRNA in each group showed that the albumin mRNA band was located at 2.1kb and the absorbance (A) nephropathy group (1.70±0.12) was higher than that of the control group (1.25±) 0.24) The treatment group (2.56 ± 0.24) was higher than the nephropathy group (P <0.01). On the basis of a protein-rich diet, Astragalus membranaceus and Angelica sinensis can upregulate liver albumin mRNA expression in rats with nephrotic syndrome, allowing rats to maintain near-in plasma albumin levels without loss of protein in daily urine. The normal range (37 ± 1.0g/L). This result suggests that by increasing the level of liver albumin mRNA in kidney disease rats, the synthesis of hepatic albumin increases, the level of plasma albumin increases, and protein metabolism is improved.