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本文采用ELISA双抗体夹心法,测定了32例无感染征象的乙型肝炎后肝硬化和30例无远处转移和恶液质的原发性肝细胞肝癌病人,在大肠杆菌内毒素诱导下,周围血单个核细胞产生肿瘤坏死因子(TNF-α)和白细胞介素6(IL-6)的能力。结果表明,肝硬化的TNF-α含量和IL-6活性水平均明显升高(P<0.001,P<0.05),应用干扰素治疗的病人,升高更为明显;肝癌的TNF-α含量下降(P<0.001),IL-6活性水平基本正常(P>0.5),与肝硬化相比,二者差异均显著(P<0.001,P<0.05)。另外,在肝癌组,90.0％有肝硬化,70.0％HBsAg阳性。结果提示,测定无感染征象的乙肝后肝硬化和没有转移及恶液质的肝癌病人周围血单个核细胞产生TNF-α、IL-6的能力,对深化肝硬化的认识具有一定意义,并有可能提示肝硬化癌变。
In this study, 32 HBV-related cirrhosis without infection and 30 patients with distant metastasis and dyscrasia with primary hepatocellular carcinoma were determined by ELISA double-antibody sandwich method. Peripheral blood mononuclear cells produce the ability to produce tumor necrosis factor (TNF-α) and interleukin 6 (IL-6). The results showed that the levels of TNF-α and IL-6 activity in liver cirrhosis were significantly higher (P<0.001, P<0.05). The increase in patients treated with interferon was more pronounced; the level of TNF-α in liver cancer decreased. (P<0.001), IL-6 activity was almost normal (P>0.5). Compared with liver cirrhosis, the difference between the two groups was significant (P<0.001, P<0.05). In addition, in the liver cancer group, 90.0% had cirrhosis and 70.0% HBsAg was positive. The results suggest that the ability to detect TNF-α and IL-6 production from peripheral blood mononuclear cells in patients with hepatic cirrhosis without hepatic infection and hepatocellular carcinoma without metastasis and dyscrasia has a certain significance for the deepening of the understanding of liver cirrhosis. It may indicate cirrhosis and canceration.