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为明确啶酰菌胺与唑胺菌酯对灰葡萄孢Botrytis cinerea毒力的增效作用,采用菌丝生长速率法测定了啶酰菌胺与唑胺菌酯不同配比混合物对灰葡萄孢菌株DZ3(对多菌灵、乙霉威和嘧霉胺高抗)的毒力,以Wadley公式评价了其协同作用。选取最佳增效组合,采用黄瓜子叶喷雾法测定了其对其他5个菌株的毒力及田间防效。通过测定菌株DZ3分生孢子悬浮液中的溶氧量、电导率和菌丝中丙二醛的含量,研究最佳增效组合的增效机制。结果表明:啶酰菌胺与唑胺菌酯不同质量比(9∶1、7∶1、5∶1、3∶1、1∶1、1∶3、1∶5、1∶7和1∶9)的混合物均对菌株DZ3表现为毒力增效作用,其中质量比为1∶3的组合增效最明显,增效系数达4.76,且对另外5个供试菌株的联合毒力也表现出增效;在使用剂量为有效成分200~300 g/hm2时,质量比为1∶3的组合对番茄灰霉病的防治效果为78.05%~93.96%,同时其对菌株DZ3的呼吸抑制作用与单剂啶酰菌胺相当,但对细胞膜通透性和菌丝膜脂质过氧化的影响均显著高于各单剂。本研究结果表明,啶酰菌胺与唑胺菌酯以质量比1∶3进行混配,可用于番茄灰霉病的防治。
In order to clarify the synergistic effect of boscalid and azadafos on the virulence of Botrytis cinerea, the mycelial growth rate method was used to determine the inhibitory effect of boscalid and azacitidil against different strains of Botrytis cinerea The toxicity of DZ3 (high resistance to carbendazim, diethylmyme and pyrimethanil) was evaluated for its synergy by the Wadley equation. The best synergistic combinations were selected and their virulence and field control efficacy on five other strains were determined by cucumber cotyledon spray method. The synergistic mechanism of the optimal synergistic combination was studied by measuring the dissolved oxygen content, the conductivity and the content of malondialdehyde in the mycelium of the strain DZ3. The results showed that the boscalid and azadirachtin different mass ratio (9: 1, 7: 1, 5: 1, 3: 1, 1: 1, 1: 3, 1: 5, 1: 9) all showed virulence synergism on strain DZ3, the synergistic effect was most obvious at the ratio of 1: 3, the synergistic coefficient reached 4.76, and the joint virulence of the other 5 tested strains also showed The results showed that the control effect on the control of tomato gray mold was 78.05% -93.96% at the dosage of 200-300 g / hm2, and the respiratory inhibition of the strain DZ3 Compared with single-dose boscalid, its effect on cell membrane permeability and mycelial lipid peroxidation were significantly higher than that of single dose. The results of this study showed that boscalid and pyraclostrobin were mixed at mass ratio of 1: 3 and could be used for the prevention and control of tomato gray mold disease.