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Objective: To investigate the role of hepatic and gas-tric alcohol dehydrogenase (ADH) in different pa-thologic stages of alcoholic liver disease (ALD).Methods: Thirty-nine Wistar rats were divided ran-domly into two groups: model group (24) and con-trol group (15). The ALD model was established byinfusing alcohol into the stomach. After hepatic andgastric tissues had been stained by enzyme histo-cyto-chemistry assay, the activity varieties of hepatic andgastric ADH were observed by an optical micro-scope, and the activity alterations were also deter-mined by LUZEX-F image analysis as a semi-quanti-tative method.Results: The activity of hepatic ADH gradually in-creased, but that of gastric ADH gradually decreasedin the different pathologic stages of alcoholic liverdisease. There was a significant difference betweenthe model group and control group (P<0.05).Conclusions: Along with occurrence of ALD, the ac-tivity of hepatic ADH gradually increased, but thatof gastric ADH gradually decreased, showing thatthe activity alterations of hepatic and gastric ADHmay play an important role in the onset and develop-ment of ALD.
Objective: To investigate the role of hepatic and gas-tric alcohol dehydrogenase (ADH) in different pa-thologic stages of alcoholic liver disease (ALD). Methods: Thirty-nine Wistar rats were divided ran-domly into two groups: model group ( 24) and con-trol group (15). The ALD model was established by infusing alcohol into the stomach. After hepatic andgastric tissues had been stained by enzyme histo-cyto-chemistry assay, the activity varieties of hepatic andgastric ADH were observed by an optical micro-scope, and the activity alterations were also deter-mined by LUZEX-F image analysis as a semi-quanti-tative method. Results: The activity of hepatic ADH gradually in-creased, but that of gastric ADH slowly decreased in the different pathologic stages of alcoholic liver disease. There was a significant difference betweenthe model group and control group (P <0.05) .Conclusions: Along with occurrence of ALD, the ac-tivity of hepatic ADH incremental increased but ased, showing that the activity alterations of hepatic and gastric ADHmay play an important role in the onset and develop-ment of ALD.