论文部分内容阅读
目的 探讨丙肝病毒核心蛋白 (HCVC蛋白 )在肝门部胆管癌发生和发展中的作用。方法 采用过氧化物酶 抗过氧化酶 (PAP)法检测 36例肝门部胆管癌组织中HCVC蛋白的表达 ,原位末端标记技术 (ISEL)和链霉亲合素 生物素技术 (SLAB)分别检测肝门部胆管癌中的细胞凋亡指数 (AI)和细胞增殖指数 (PI)。结果 36例肝门部胆管癌患者中HCVC蛋白阳性表达率为61 .1 % (2 2 /36) ,AI和PI的平均值分别为 (3 .46± 0 .62 ) %和 (46 .43± 1 2 .59) % ,HCVC蛋白阳性表达组中AI明显低于HCVC蛋白阴性表达组 (P <0 .0 1 ) ,而PI在HCVC蛋白阳性组中明显高于HCVC蛋白阴性组 (P <0 .0 1 )。结论 HCVC蛋白可能有助于肝门部胆管癌细胞增殖 ,抑制细胞凋亡的发生率
Objective To investigate the role of hepatitis C virus core protein (HCVC) in the occurrence and development of hilar cholangiocarcinoma. Methods The expression of HCV C protein in 36 cases of hilar cholangiocarcinoma was detected by peroxidase-peroxidase (PAP) method. ISEL and SLAB Apoptosis index (AI) and cell proliferation index (PI) in hilar cholangiocarcinoma were detected. Results The positive rate of HCVC protein in 36 cases of hilar cholangiocarcinoma was 61.1% (2/36), and the average values of AI and PI were (3.46 ± 0.62)% and (46.43 ± 1 2 .59%). The AI in HCVC protein positive group was significantly lower than that in HCVC protein negative group (P <0.01), while PI was significantly higher in HCVC protein positive group than in HCVC protein negative group (P < 0 .0 1). Conclusion HCVC protein may contribute to the proliferation of hilar cholangiocarcinoma cells and inhibit the incidence of apoptosis