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目的对高效液相色谱法检测清开灵注射液中黄芩苷与绿原酸含量的效果进行分析探讨,为今后的药学研究工作提供可靠的参考依据。方法固定相为C18硅胶柱,流动相为甲醇-0.4%磷酸水溶液,梯度洗脱,流速1.0ml/min,波长为323nm。结果绿原酸的线性范围4.251～105.900μg/ml,r=0.9998,黄芩苷的线性范围9.223～203.700μg/ml,r=0.9999。实验的重现性、精密度良好,样品稳定性理想。结论经高效液相色谱法对清开灵注射液中黄芩苷与绿原酸含量进行检测具有操作简单、快速、结果准确可靠、精密度与重现性理想,在清开灵注射液质量控制中具有重要作用,值得关注。
Objective To analyze the effect of HPLC on the determination of baicalin and chlorogenic acid in Qingkailing Injection, and provide a reliable reference for the future research of pharmacy. Methods The stationary phase was C18 silica gel. The mobile phase consisted of methanol-0.4% phosphoric acid solution with gradient elution at a flow rate of 1.0 ml / min and a wavelength of 323 nm. Results The linear range of chlorogenic acid was 4.251 ~ 105.900μg / ml, r = 0.9998. The linear range of baicalin was 9.223 ~ 203.700μg / ml, r = 0.9999. Experimental reproducibility, good precision, good sample stability. Conclusion The detection of baicalin and chlorogenic acid in Qingkailing injection by HPLC has the advantages of simple and rapid operation, accurate and reliable results, good precision and reproducibility. In the quality control of Qingkailing Injection, Has an important role, it is worth attention.