【目的】探讨不同基因对不同棉花品种的遗传转化差异。【方法】以新海14号、新海16号、新海24号和新海30号四个不同基因型海岛棉胚性愈伤组织为受体材料,通过农杆菌介导法分别将pBI121-bar/Bt和pBI121-Bt转化至四个品种中,对遗传转化体系中的PPT浓度和Cef浓度进行筛选,观察比较不同浓度下不同基因型胚性愈伤的生长状况,并对单价表达载体和双价表达载体转化不同基因型胚性愈伤的生长状态进行比较。【结果】转化体系的筛选确定双价表达载体的PPT筛选浓度为3.0 mg/L,Cef的筛选浓度为800 mg/L;转化单价表达载体LBA4404/pBI121-Bt的Cef的筛选浓度为600 mg/L。转化单价表达载体后,新海14号的出愈率最高,新海30号的出愈率最低;转化双价表达载体后,新海14号的出愈率最高,新海24号的出愈率最低。【结论】不同基因型对PPT的敏感性有差异;转化不同基因后,胚性愈伤对抗生素的敏感度有所不同;转化相同基因后,不同品种的胚性愈伤生长存在差异。 【Objective】 The purpose of this study was to investigate the genetic transformation of different cotton varieties. 【Method】 Embryogenic embryos of four island genotypes of island cotton (Xindao No.14, Xinhai No.16, Xinhai No.24 and Xinhai No.3) were used as materials. Agrobacterium tumefaciens-mediated transformation of pBI121-bar / Bt and pBI121-Bt was transformed into four cultivars, and the PPT concentration and Cef concentration of the genetic transformation system were screened. The growth status of embryogenic callus of different genotypes under different concentrations was observed and compared, and the monovalent expression vector and the bivalent expression vector Transformation of different genotypes of embryogenic callus growth status were compared. 【Result】 The screening system of transformation system confirmed that the screening concentration of PPT was 3.0 mg / L and the screening concentration of Cef was 800 mg / L. The screening concentration of Cef transformed with monovalent expression vector LBA4404 / pBI121-Bt was 600 mg / L. After transformation of monovalent expression vector, Xinhai 14 had the highest out-of-cure rate and Xinhai 30 with the lowest out-of-cure rate. After transformation of bivalent expression vector, Xinhai 14 had the highest out-of-cure rate and Xinhai 24 with the lowest out-of-cure rate. 【Conclusion】 The sensitivity of different genotypes to PPT is different. The sensitivity of embryogenic callus to antibiotics is different when different genes are transformed. The embryogenic callus growth of different varieties is different after the same gene is transformed.