论文部分内容阅读
目的从人胎盘组织中分离培养获得间充质干细胞(MSCs),并进一步研究其对异体T淋巴细胞细胞因子白细胞介素2(IL-2)、γ-干扰素(IFN-γ)分泌的影响。方法首先从胎盘组织中分离培养胎盘间充质干细胞(PM-SCs),在体外观测其形态,并通过细胞表面抗原表达、分化潜能等特征进行鉴定;然后将体外分离培养扩增的PMSCs按照不同比例加入双向混合淋巴细胞培养体系(MLR)中,共同培养3d后,用ELISA法检测各组MLR上清中细胞因子IL-2与IFN-γ的含量。结果从人胎盘组织中分离培养获得MSCs,具有与骨髓间充质干细胞(BMSCs)极为相似的细胞形态及细胞表面标志,并且还具有与BMSCs相似的跨胚层分化能力,能在一定条件下被诱导分化出神经元样细胞。PMSCs与同种异体混合淋巴细胞共同培养后,能有效抑制MLR中T淋巴细胞细胞因子IL-2与IFN-γ的分泌(P<0.05)。结论胎盘组织是MSCs的有效来源,PMSCs具有与BMSCs相似的生物学特性及免疫调节机制,为MSCs的研究提供了又一重要的细胞来源。
OBJECTIVE: To isolated and culture mesenchymal stem cells (MSCs) from human placenta and further investigate their effects on the secretion of cytokines interleukin 2 (IL-2) and interferon gamma (IFN-γ) from allogeneic T lymphocytes . Methods Firstly, placental mesenchymal stem cells (PM-SCs) were isolated and cultured from placenta and their morphology was observed in vitro. The cell surface antigen expression and differentiation potential were identified. Then, the cultured PMSCs were isolated and cultured in different ways (MLR). After cultured for 3 days, the contents of IL-2 and IFN-γ in supernatants of MLR in each group were detected by ELISA. Results MSCs were isolated and cultured from human placenta. MSCs had the morphological and cell surface markers closely related to bone marrow mesenchymal stem cells (BMSCs), and also had the ability to differentiate into mesoderm and BMSCs, which could be induced under certain conditions Differentiation of neuron-like cells. PMSCs and allogeneic mixed lymphocytes co-cultured can effectively inhibit the secretion of cytokines IL-2 and IFN-γ of T lymphocytes in MLR (P <0.05). Conclusion Placenta tissue is an effective source of MSCs. PMSCs have similar biological characteristics and immunoregulatory mechanisms as BMSCs, which provide another important source of cells for the study of MSCs.