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sHLA-G是妊娠期特异的免疫抑制分子,在受孕子宫及妊娠期女性外周血中含量丰富,提示其在维持母胎耐受中发挥重要作用。我们前期研究发现:早期难免流产病人组蜕膜组织KIR2DL4、ILT-2、ILT-4分子表达的水平明显低于正常妊娠对照组。为进一步分析这些抑制性受体表达下降与sHLA-G是否存在联系,我们对sHLA-G与蜕膜组织免疫细胞NKR表达的关系进行了研究。我们将不同量的sHLA-G1、sHLA-G2蛋白加至蜕膜单个核细胞悬液、外周血单个核细胞悬液及NK92细胞株中,分别孵育12h、24h、48h后,用Real-Time RT-PCR、FACS对NKR的表达进行检测。研究发现sHLA-G可以不同程度地上调蜕膜单个核细胞、外周血单个核细胞、NK92细胞表面ILT-2、ILT-4、KIR2DL4的表达。这个发现让我们对sHLA-G诱导免疫耐受的机制有了更深的认识。sHLA-G上调抑制性受体表达这一作用具有重要生理意义,它可以抑制效应细胞的杀伤功能,使母胎界面微环境适于胎儿存活,从而维持母胎免疫耐受。
sHLA-G is a specific immunosuppressive molecule during pregnancy. It is abundant in the peripheral blood of pregnant women and pregnant women, suggesting that sHLA-G plays an important role in the maintenance of maternal-fetal tolerance. Our previous study found that the level of KIR2DL4, ILT-2 and ILT-4 expression in decidua tissue of patients with early unavoidable abortion was significantly lower than that of normal pregnancy control group. In order to further analyze whether there is a relationship between sHLA-G and sHLA-G, the relationship between sHLA-G and the NKR expression in immune cells of decidua was studied. We added different amounts of sHLA-G1 and sHLA-G2 proteins to decidual mononuclear cell suspension, peripheral blood mononuclear cell suspension and NK92 cell line and incubated them for 12h, 24h and 48h, respectively. Real-Time RT The expression of NKR was detected by PCR and FACS. The study found that sHLA-G up-regulated the expression of ILT-2, ILT-4 and KIR2DL4 on decidual mononuclear cells, peripheral blood mononuclear cells and NK92 cells to varying degrees. This finding gives us a deeper understanding of the mechanisms by which sHLA-G induces immune tolerance. sHLA-G upregulates the expression of inhibitory receptor has an important physiological significance, it can inhibit the killing effect of effector cells, maternal-fetal interface microenvironment suitable for fetal survival, so as to maintain the immune tolerance of the mother’s fetus.