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WD40转录因子也称WDR(WD repeat)蛋白,是一种古老蛋白家族,参与调节类黄酮次生代谢途径中多种酶基因的表达,从而影响类黄酮的积累。以三年生银杏苗为试材,采用RT-PCR和RACE-PCR方法,研究了银杏WD40基因序列,以期深入了解银杏叶类黄酮合成代谢的分子机理。结果表明:GbWD401序列包含一段2 313bp的开放阅读框,编码一个770aa的肽链;GbWD402序列包含一段1 605bp的开放阅读框,编码一个534aa的肽链。银杏GbWD401和GbWD402基因推测编码蛋白序列均含有典型WD40重复基序。GbWD401蛋白序列与无油樟AtWD401相似性为49%,GbWD402蛋白序列与无油樟AtWD402相似性为78%。系统进化树发现,GbWD401与无油樟AtWD401、荷花NnWD40划归为同一分支;而GbWD402与无油樟AtWD402划归为另一分支。
WD40 transcription factor, also known as WDR (WD repeat) protein, is an ancient protein family involved in regulating the expression of multiple enzyme genes in flavonoid secondary metabolic pathways that affect flavonoid accumulation. The Ginkgo biloba seedlings were used as materials to study the molecular mechanism of Ginkgo biloba WD40 gene by RT-PCR and RACE-PCR methods. The results showed that the GbWD401 sequence contained a 2 313 bp open reading frame and encoded a 770aa peptide. The GbWD402 sequence contained a 1 605 bp open reading frame and encoded a 534aa peptide. Ginkgo GbWD401 and GbWD402 genes presumed encoded protein sequence contains a typical WD40 repeat motif. The similarity of GbWD401 protein sequence to AtWD401 was 49%. The similarity of GbWD402 protein sequence to AtWD402 was 78%. Phylogenetic analysis revealed that GbWD401 was attributed to AtWD401 and NnWD40, while GbWD402 and AtWD402 were classified as another branch.