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以从白菜软腐病根部土壤分离获得的1株能拮抗细菌性软腐病的拮抗真菌为试材,以平板对峙培养其抑菌圈达(37.7±2.2)mm,白菜离体叶片防效可达75%,依据形态特征结合rDNA-ITS序列分析,将获得的拮抗真菌鉴定为草茎点霉,并命名为Phoma herbarum F217-1,并研究了其生物学特性。结果表明:该菌株在马铃薯蔗糖培养基(PSA)上生长最佳,碳源和氮源以葡萄糖和硝酸钠较适宜,生长适温为28℃,致死温度为55℃,在pH 3~10条件下均生长良好,其最适pH 5~8,通气条件对草茎点霉菌丝生长均无明显影响,但光照24h条件下菌丝生长较差。
Antagonistic fungi antagonistic to bacterial soft rot were obtained from the soils of the roots of cabbage soft rot, and the zone of inhibition was reached (37.7 ± 2.2) mm with plate confrontation. Up to 75%. Based on the morphological characteristics and rDNA-ITS sequence analysis, the antagonistic fungi identified were identified as Phoma herbarum F217-1 and its biological characteristics were studied. The results showed that the strain grew best on potato sucrose medium (PSA). The optimal carbon source and nitrogen source were glucose and sodium nitrate. The suitable growth temperature was 28 ℃ and the lethal temperature was 55 ℃. Under the conditions of pH 3 ~ 10 Under the optimum conditions, the optimum pH was 5 ~ 8. The aeration conditions had no significant effect on the mycelial growth of A. philoxeroides, but the mycelial growth was poor under 24h illumination.