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目的:探讨远距缺血后适应(RIP)对兔局部短期缺血再灌注心肌的保护作用。方法:将24只新西兰白兔随机平均分成4组:假手术对照组(S组)、缺血再灌注对照组(IR组)、缺血后适应组(Post组)、RIP组。采用TUNEL分析检测各组心肌组织的细胞凋亡情况,Western blot方法检测Bcl-2、Bax蛋白的表达。结果:RIP组心肌细胞凋亡指数[30.24±1.68)%]显著低于IR组[(53.30±12.88)%],P<0.01;Post组与RIP组心肌凋亡指数相比差异无统计学意义。Bcl-2基因的蛋白表达量RIP组高于IR组(0.97±0.16∶0.77±0.14,P<0.05);Bax基因的蛋白表达量RIP组低于IR组(0.76±0.18∶1.09±0.13,P<0.05)。Bcl-2、Bax基因的蛋白表达量在RIP组及Post组相比差异无统计学意义。结论:RIP显著减少了兔短期缺血再灌注诱导的心肌细胞凋亡程度与上调Bcl-2基因的蛋白表达,下调Bax基因的蛋白表达有关,与Post一样具有心肌保护作用。
Objective: To investigate the protective effects of remote ischemic postconditioning (RIP) on myocardial ischemia and reperfusion in rabbits. Methods: Twenty-four New Zealand white rabbits were randomly divided into 4 groups: sham operation control group (S group), ischemia reperfusion control group (IR group), post-ischemic adaptation group (Post group) and RIP group. TUNEL assay was used to detect the apoptosis of myocardial tissue in each group. Western blot was used to detect the protein expression of Bcl-2 and Bax. Results: The apoptotic index of cardiomyocytes in RIP group was significantly lower than that in IR group [(30.34 ± 1.68)%] [(53.30 ± 12.88)%], P <0.01; There was no significant difference in the apoptotic index between RIP group and Post group . The protein expression of Bcl-2 in RIP group was significantly higher than that in IR group (0.97 ± 0.16:0.77 ± 0.14, P <0.05). The protein expression of Bax in RIP group was lower than that in IR group (0.76 ± 0.18:1.09 ± 0.13, P <0.05). The protein expression of Bcl-2 and Bax was no significant difference between RIP group and Post group. CONCLUSION: RIP significantly reduces myocardial apoptosis induced by short-term ischemia-reperfusion in rabbits, which is related to upregulation of Bcl-2 gene expression and down-regulation of Bax gene protein expression, and myocardial protection as well as Post.