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生长素响应因子(auxin response factor,ARF)基因家族是植物特有的转录因子家族,在调控植物生长发育过程中起重要作用。以公布的甘蓝基因组数据库为基础,采用生物信息学方法鉴定出29个ARF基因,命名为BoARFs,研究分析了其基因结构、染色体分布、蛋白质保守结构域、系统进化树及表达模式,以期为甘蓝ARF基因家族功能的深入研究提供参考依据。结果表明:BoARF家族基因结构相对复杂,含有2~14个外显子;在染色体上呈不均匀分布,除BoARF29基因未定位到染色体上外,其它基因均定位在不同的染色体上;所有BoARF蛋白均具有保守的B3结构域和Auxin_resp结构域,但只有21个BoARF蛋白包含1~2个AUX/IAA结构域;转录组数据表达模式分析表明,ARF基因具有不同的组织表达模式,部分基因表现出组织特异性。
The auxin response factor (ARF) gene family is a plant-specific transcription factor family that plays an important role in the regulation of plant growth and development. Based on the published database of cabbage genomes, 29 ARF genes were identified by bioinformatics method and named as BoARFs. The gene structure, chromosome distribution, conserved protein domains, phylogenetic tree and expression pattern of Brassica napus ARF gene family function in-depth study provide a reference. The results showed that the BoARF family gene structure is relatively complex and contains 2 to 14 exons; it is unevenly distributed on the chromosome. All other genes are located on different chromosomes except BoARF29 gene is located on chromosomes. All BoARF proteins But only 21 BoARF proteins contained 1 ~ 2 AUX / IAA domains. The analysis of transcriptome data showed that ARF genes had different tissue expression patterns, and some of the genes showed Tissue specificity.