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以昆虫病毒Flockhousevirus(FHV)外壳蛋白为载体的外源抗原表位表达系统(FHV-RNA2载体系统).在重组杆状病毒和重组pET系统中构建和表达了SA11Vp4胰酶切割位点两侧和重叠切割位点3个抗原表位氨基酸序列(抗原表位A,aa223~242;抗原表位B,aa243~262;抗原表位C,aa234~251),并对其免疫原性进行了研究。结果表明:这3个抗原表位能诱导动物产生抗同源氨基酸序列的抗体和抗同源病毒(SA11)感染性的血清中和抗体。研究结果提示:RVVp4胰酶切割位点区氨基酸序列除了具有胰酶切割增强病毒感染力外,还具有诱导动物机体产生血清中和抗体的能力,是RV重组抗原表位亚单位疫苗研究中重要的抗原表位氨基酸序列。
Exogenous antigen epitope expression system (FHV-RNA2 vector system) using the insect virus Flockhousevirus (FHV) coat protein as a carrier. Amino acid sequences of three epitopes (epitope A, aa223 ~ 242; epitope B, aa243) were constructed and expressed in the recombinant baculovirus and recombinant pET system on both sides of the cleavage site of SA11Vp4 and overlapping cleavage sites ~ 262; antigenic epitope C, aa234 ~ 251), and its immunogenicity was studied. The results showed that these three epitopes can induce the animals to produce anti-homologous amino acid sequence antibodies and anti-homologous virus (SA11) sero-neutralizing antibodies. The results suggest that the amino acid sequence of RVVp4 pancreatic enzyme cleavage site has the ability of inducing serum neutralizing antibody in the animal body in addition to pancreatic enzyme cutting to enhance the virulence of the virus and is important in the research of RV recombinant epitope vaccine Epitope amino acid sequence.