论文部分内容阅读
[目的]探讨肿瘤坏死因子TNF-β+252基因多态性与肺癌遗传易感性的关系。[方法]采用聚合酶链式反应-限制性片段长度多态性(PCR-RELP)方法对152例肺癌患者(病例组)和127例健康者(对照组)的TNF-β+252基因型进行分析。[结果]TNF-β+252基因型频率和等位基因分布频率在肺癌组和对照组比较差异均无统计学意义(χ2=0.60,P=0.74;χ2=0.24,P=0.63)。分层分析发现,不吸烟人群中肺癌组和对照组基因型分布差异有统计学意义(χ2=6.67,P=0.04),与AA基因型相比GG基因型增加了肺癌的患病风险(OR:3.97,95%CI:1.34～11.79),而等位基因的分布频率在两组之间差异无统计学意义(χ2=1.54,P=0.22)。[结论]TNF-β+252多态性与肺癌易感性相关,不吸烟人群中GG基因型增加了肺癌的发病风险。
[Objective] To investigate the relationship between TNF-β + 252 gene polymorphism and genetic susceptibility to lung cancer. [Method] The genotypes of TNF-β + 252 in 152 patients with lung cancer (case group) and 127 healthy controls (control group) were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP) analysis. [Results] The frequencies of genotypes and alleles of TNF-β + 252 were not significantly different between lung cancer group and control group (χ2 = 0.60, P = 0.74; χ2 = 0.24, P = 0.63). Stratified analysis showed that the distribution of genotypes in lung cancer group and control group was statistically significant (χ2 = 6.67, P = 0.04) in non-smokers. Compared with AA genotype, GG genotype increased the risk of lung cancer (OR : 3.97, 95% CI: 1.34 ~ 11.79). However, there was no significant difference in allele frequencies between the two groups (χ2 = 1.54, P = 0.22). [Conclusion] The polymorphism of TNF-β + 252 is associated with the susceptibility to lung cancer. The GG genotype in non-smokers increased the risk of lung cancer.