采用双波长分光光度法测定了大鼠小肠循环液中酚红的含量 ,方法的线性范围为 1～ 8mg/L ,回收率为 99 88%～ 99 99% (n =5 ) ,日内RSD为 0 5 1%～ 1 2 7% (n =4) ,日间RSD为0 5 8%～ 2 2 9% (n =4) . The method of dual wavelength spectrophotometry was used to determine the content of phenol red in the small intestine fluid of rats. The linear range was 1 ~ 8mg / L, the recovery was 99 88% ~ 99 99% (n = 5) 5 1% -12 27% (n = 4), daytime RSD was 0 58% -2 229% (n = 4).