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为了解EB病毒基因组阳性人鼻咽癌DNA在转染正常细胞后可能发生的生物学作用,作者将人鼻咽癌活检组织的DNA(EB病毒基因组阳性),用磷酸钙方法转染正常Rat-Ⅰ细胞,使其恶转;用软琼脂培养的转化细胞能贴壁生长,并形成克隆;转化细胞易与PHA发生凝集;克隆接种裸鼠形成纤维肉瘤;转化细胞和裸鼠肿瘤组织用抗C_3补体免疫荧光法检测EBNA呈阳性;染色体原位杂交显示在某些细胞中期染色体上有银粒出现。
In order to understand the biological role of Epstein-Barr virus genome-positive nasopharyngeal carcinoma DNA in transfecting normal cells, the authors transcribe the DNA of human nasopharyngeal carcinoma biopsy tissue (Epstein-Barr virus genomic positive) into normal Rat- Ⅰ cells were transformed to malignant transformation. Transformed cells cultured on soft agar could grow in adherent growth and form clones. The transformed cells were easily aggregated with PHA. The colonies were inoculated with nude mice to form fibrosarcoma. The transformed cells and the nude mice were treated with anti-C_3 EBNA was detected by complement immunofluorescence. Chromosome in situ hybridization showed silver grains appeared in some metaphase chromosomes.