论文部分内容阅读
目的:研究脐带间质干细胞的多向分化潜能,为心血管组织工程研究提供一个新的种子细胞来源。方法:采用胶原酶胰酶消化脐带组织,将获得的细胞传代培养,流式细胞仪鉴定细胞表面分子,再以不同方法使其向骨、脂肪、心肌和内皮细胞方向诱导,分别采用Von Kossa、油红O染色和免疫组化对分化的细胞进行鉴定。结果:脐带间质干细胞分别向成骨细胞和成脂肪细胞诱导培养后,Von Kossa染色和油红O染色阳性。在5-氮胞苷诱导后,心肌特异性抗体肌动蛋白α-actin和肌球蛋白myosin免疫组化染色阳性。在VEGF诱导后,细胞形成网格样结构,免疫荧光示CD31、CD34染色阳性。结论:脐带间质干细胞具有分化为骨、脂肪、心肌和内皮细胞的潜能,是非常有前途的心血管组织工程种子细胞来源。
Objective: To investigate the multidirectional differentiation potential of umbilical cord mesenchymal stem cells and to provide a new source of seed cells for cardiovascular tissue engineering. Methods: Umbilical cord tissue was digested with collagenase and subcultured. The cell surface molecules were identified by flow cytometry, and then induced into bone, fat, myocardium and endothelial cells by different methods. Von Kossa, Differentiated cells were identified by Oil Red O staining and immunohistochemistry. Results: Umbilical cord mesenchymal stem cells were cultured in osteoblasts and adipocytes respectively. Von Kossa staining and oil red O staining were positive. After 5-azacytidine induction, myocardial specific antibodies actin α-actin and myosin immunohistochemical staining. After VEGF induction, the cells formed a grid-like structure, immunofluorescence showed CD31, CD34 staining positive. CONCLUSION: Umbilical cord mesenchymal stem cells have the potential to differentiate into bone, fat, myocardium and endothelial cells and are a very promising source of seed cells for cardiovascular tissue engineering.