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洋葱黄矮病毒(Onion yellow dwarf virus,OYDV)是危害洋葱质量和产量的主要病毒之一.本研究从吉林省农安县的分蘖洋葱种植区分离到一株OYDV,通过RT-PCR克隆OYDV衣壳蛋白(coat protein,cp)基因,构建重组质粒pET28-OY-DV-cp,转化大肠杆菌Rosetta,经诱导后表达并纯化,Western-blot鉴定为OYDV cp蛋白.与实验室制备的9株大豆花叶病毒单克隆抗体进行反应,得出其中3株单克隆抗体(2D3、4F9、4G12)能够识别OYDV.综上,本研究首次从分蘖洋葱上分离并鉴定OYDV,对OYDV cp基因进行原核表达,获得重组蛋白,并且鉴定到能够识别OYDV的单克隆抗体3株,为分蘖洋葱病毒检测提供了抗体材料.“,”Onion yellow dwarf virus (OYDV) is one of the major viruses that damage the quality and yield of shallot. In this study, OYDV was isolated from the tiller onion plantation in Nongan County, Jilin Province, and the OYDV cp gene was cloned by RT-PCR. The recombinant plasmid pET28-OYDV-cp was constructed and transformed into Escherichia coli strain Rosetta. Then cp was expressed and purified after induction and was identified as OYDV cp protein by Western-blot. The reaction analysis of OYDV cp protein with 9 monoclonal antibodies against Soybean mosaic virus showed that three monoclonal antibodies (2D3, 4F9, 4G12) could recognize specifically OYDV. In summary, OYDV cp was isolated from the tiller onion for the first time. The recombinant cp was obtained by prokaryotic expression, and three strains of monoclonal antibody capable of recognizing OYDV were identified, which provided antibody material for the detection of OYDV.