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从80个随机引物中筛选到带型清晰、多态性及重复性均好的10个引物,对采自广东省1998-1999年四个自然生态稻作区的101个稻瘟病菌菌株进行随机扩增多态性DNA (Random Amplified Polymorphic DNA, RAPD) 指纹分析。10个引物共扩增出113条多态性带,表明广东省稻瘟病菌具有丰富的遗传多样性;RAPD分析可为该菌的遗传多样性分析提供大量的分子标记。对菌株间相似性系数和应用加权算术平均组对法 (Unweighted Pair Group Method using Arithmetic Average, UPGMA) 构建的聚类树状图进行分析,以相似性系数为0.62阀值时,可将101个菌株划分为14个遗传宗谱;其中宗谱1及宗谱2的菌株数占总数的80.2%,为优势宗谱; 其余的20个菌株分别归属于其他12个宗谱,由此说明广东省的稻瘟病病原菌群体既存在很突出的优势宗谱,又存在较多具遗传多样性的小宗谱。分析不同稻作生态区的菌株发现,每个稻作生态区既有共同的宗谱,又有其特异的宗谱;广东省稻瘟病菌群体遗传多样性的组成在不同生态稻作区是相对地比较稳定的。分析不同年份和早晚稻生长季节采集的菌株发现,广东省稻瘟病菌群体遗传多样性在年份和早晚稻生长季节之间也存在一定的特异性。
A total of 101 random primers with clear genotypes, polymorphisms and reproducibility were screened from 80 random primers and 101 strains of M. grisea strains collected from four natural ecological rice growing regions of Guangdong Province during 1998-1999 Random Amplified Polymorphic DNA (RAPD) fingerprinting. A total of 113 polymorphic bands were amplified by 10 primers, which indicated that M. grisea in Guangdong Province was rich in genetic diversity. RAPD analysis provided a large number of molecular markers for genetic diversity analysis of this strain. The clustering dendrogram constructed based on the similarity coefficient between strains and using the UPGMA (Unweighted Pair Group Method using Arithmetic Average) method was analyzed. When the similarity coefficient was 0.62, 101 strains Divided into 14 genetic lineage; which lineage 1 and lineage 2 accounted for 80.2% of the total number of strains, the dominant genealogy; the remaining 20 strains belong to the other 12 genealogy, which shows that Guangdong There are two prominent genealogies for the pathogen of rice blast and a large number of genealogical minor genealogies. The analysis of the strains in different rice ecological regions found that each rice ecological region had both a common genealogy and a specific genealogy. The composition of genetic diversity of rice blast germplasm in Guangdong Province was relatively Relatively stable. Analysis of strains collected in different years and early and late rice growing season found that the genetic diversity of M. grisea populations in Guangdong also had certain specificity between the year and the growing season of early and late rice.