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目的使用人参对照提取物(Ginseng Extract Reference Substance,GS ERS)对人参样品进行定性鉴别及定量分析。方法使用薄层色谱法、硅胶高效薄层板,以氯仿-乙酸乙酯-甲醇-水(15∶40∶22∶10)10℃以下分层的下层溶剂为展开剂,对人参样品进行定性鉴别。采用Kromasil 100-5 C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水,梯度洗脱;流速1.0 m L·min~(-1),柱温25℃,检测波长203 nm,进样量10μL。用已知含量的GS ERS外标法测定人参样品中人参皂苷Rg_1、Re、Rf、Rb_1、Rc、Rb_2、Rd 7个成分的含量。结果高效薄层色谱显示GS ERS与人参药材图谱具有一致性,且与西洋参有明显区别;使用GS ERS外标法测定人参及西洋参样品中的人参皂苷成分,结果与使用化学对照品外标法测定结果一致,两种方法相对平均偏差在0.02%~2.90%之间。结论 GS ERS用于人参样品的定性鉴别以及人参药材中人参皂苷Rg1,Re,Rf,Rb1,Rc,Rb2,Rd 7个成分的含量测定,方便有效,经济实惠。对照提取物符合中药整体性和复杂性的特点,更适合中药整体质量控制。
Objective To identify and quantify ginseng samples by using Ginseng Extract Reference Substance (GS ERS). Methods Thin layer chromatography (TLC) and silica gel high performance thin layer plate were used to qualitatively identify ginseng samples with the lower layer solvent of chloroform-ethyl acetate-methanol-water (15:40:22:10) . The mobile phase was acetonitrile-water with a gradient of 1.0 mL · L -1 on a Kromasil 100-5 C 18 column (4.6 mm × 250 mm, 5 μm) Wavelength 203 nm, injection volume 10μL. The content of ginsenoside Rg_1, Re, Rf, Rb_1, Rc, Rb_2, Rd in ginseng samples was determined by GS ERS external standard method with known content. Results High-performance thin layer chromatography showed that GS ERS was consistent with that of Panax ginseng and was significantly different from that of American ginseng. GSSR was used to determine ginsenosides in ginseng and American ginseng samples. The results were in agreement with the external standard of chemical reference substance The results are consistent, the relative average deviation of the two methods is between 0.02% ~ 2.90%. Conclusion The method of GS ERS for the qualitative identification of ginseng samples and the determination of seven components of ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2 and Rd in ginseng are convenient, effective and economical. Control extract in line with the characteristics of traditional Chinese medicine integrity and complexity, more suitable for the overall quality control of traditional Chinese medicine.