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目的:研究苦参总碱对溃疡性结肠炎模型大鼠的治疗作用及对核因子-κB、下游炎症细胞因子表达的影响。方法:采用TNBS(2,4,6-三硝基苯磺酸)+乙醇法制备大鼠溃疡性结肠炎(ulcerative colitis)模型,随机分为模型对照组、苦参总碱低、中、高剂量组(100、200、400 mg/kg)、阳性对照组(柳氮磺胺吡啶,Sulfasalazine)等5组,另设正常对照组大鼠10只。留取结肠组织标本,观察结肠大体形态变化,并做病理组织学检查。采用酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)法观察溃疡性结肠炎模型大鼠血清中肿瘤坏死因子-α(TNF-α)、白介素-8(IL-8)和白介素-1(IL-1)的含量,采用Western blot法检测结肠组织NF-κB的表达水平。结果:与模型组相比,苦参总碱100 mg/kg组能改善溃疡性结肠炎模型大鼠结肠大体形态评分,减轻肠道炎性反应和粘膜损伤;苦参总碱(100、200、400 mg/kg)干预后能降低血清中炎症因子TNF-α、IL-1和IL-8的水平。苦参总碱治疗组均能下调结肠组织中NF-κB的蛋白表达,呈现的剂量依赖性。结论:苦参总碱能够抑制NF-κB的活化,减少炎性因子表达,对溃疡性结肠炎有明显的治疗作用。
Objective: To investigate the therapeutic effect of matrine alkaloids on the ulcerative colitis model rats and its effect on the expression of nuclear factor-κB and downstream inflammatory cytokines. Methods: The ulcerative colitis model was prepared by TNBS (2,4,6-trinitrobenzene sulfonic acid) + ethanol method and randomly divided into model control group, low, medium and high Dose group (100, 200, 400 mg / kg), positive control group (sulfasalazine) and other 5 groups, and another 10 normal control rats. Colon tissue samples were taken to observe the general morphological changes of the colon, and histopathological examination. The levels of tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8) and interleukin-1 (IL-8) in serum of rats with ulcerative colitis were measured by enzyme-linked immunosorbent assay (ELISA) (IL-1) in colon tissue and Western blot was used to detect the expression of NF-κB in colon tissue. Results: Compared with the model group, the 100 mg / kg matrine alkaloids group could improve the colonic morphology of ulcerative colitis rats and relieve intestinal inflammatory reaction and mucosal damage. The total alkaloids of matrine (100, 200, 400 mg / kg) could decrease the levels of TNF-α, IL-1 and IL-8 in serum. The kushen total alkaloids treatment group can down-regulate the expression of NF-κB in colonic tissue in a dose-dependent manner. Conclusion: Sophora alkaloids can inhibit the activation of NF-κB, reduce the expression of inflammatory cytokines and have a significant therapeutic effect on ulcerative colitis.