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目的探索不同浓度的葡萄糖对铜绿假单胞菌生物膜形成能力的影响。方法用含有不同葡萄糖浓度的胰蛋白胨大豆肉汤培养铜绿假单胞菌,测定其570 nm处光密度,通过函数Linest进行线性回归拟合,用激光扫描共聚焦显微镜(CLSM)观察不同浓度葡萄糖培养铜绿假单胞菌生物膜的形成过程及厚度。结果 22株细菌不同浓度葡萄糖的A_(570)拟合直线的斜率均大于0,其中4株菌上升趋势明显。与无糖组及低浓度葡萄糖组相比,当葡萄糖浓度≥11.1mmol·L~(-1)时,生物膜成熟速度加快、成熟时间减少、粘附能力增强、厚度增加(P<0.05)。结论葡萄糖浓度≥11.1 mmol·L~(-1)时对铜绿假单胞菌生物膜形成有明显的诱导作用,并且随葡萄糖浓度的升高,诱导作用增强。
Objective To explore the effects of different concentrations of glucose on the biofilm formation ability of Pseudomonas aeruginosa. Methods Pseudomonas aeruginosa was cultured in tryptic soy broth containing different glucose concentrations. The optical density at 570 nm was measured and linear regression fit was performed by Linest. Laser scanning confocal microscopy (CLSM) was used to observe the effects of different concentrations of glucose Formation process and thickness of Pseudomonas aeruginosa biofilm. Results The slopes of A 570 lines of 22 strains of bacteria with different concentrations of glucose were all greater than 0, of which 4 strains showed obvious upward trend. Compared with the glucose-free group and the low-glucose group, the biofilm maturation rate was accelerated, the maturation time was reduced, the adhesion ability was enhanced, and the thickness was increased (P <0.05) when the glucose concentration was ≥11.1mmol·L -1. Conclusion The biofilm formation of Pseudomonas aeruginosa was induced obviously when the concentration of glucose was more than or equal to 11.1 mmol·L -1, and the induction was enhanced with the increase of glucose concentration.