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本文以水稻品种广陆矮4号为材料,在颖花原基分化期、花粉母细胞减数分裂期和花粉粒形成期分别采用浸根、注射和喷施3种方法引入Na_2~(14)CO_3,剂量222×10~4Bq/株。M1代根尖染色体畸变细胞率增加,花粉母细胞减数分裂期注射Na_2~(14)CO_3处理的染色体畸变细胞率最高,其次是花粉粒形成期喷施Na_2~(14)CO_3处理。在负极区Rf0.64—0.82出现新的过氧化物酶同工酶带。同时还发现,染色体畸变细胞率高的处理组新增的过氧化物酶同工酶带活性减弱,其他处理组新酶带的活性较强,说明~(14)C作用机制的复杂性。
In this paper, the rice variety Guangluan 4 was used as material to introduce Na_2 ~ (14) into three kinds of methods, ie dipping roots, injecting and spraying, respectively during the stages of spike primordium differentiation, meiosis of pollen mother cells and pollen grains formation. CO_3, the dose of 222 × 10 ~ 4Bq / strain. The chromosome aberration rate of M1 chromosome was increased, and the chromosome aberration rate of the cells treated with Na_2 ~ (14) CO_3 was the highest at meiotic stage of pollen mother cells, followed by the treatment with Na_2 ~ (14) CO_3 at pollen grains formation. A new peroxidase isozyme band appeared in the negative zone Rf 0.64-0.82. At the same time, it was also found that peroxidase isoenzyme band activity of the treated group with high chromosome aberration rate was weakened, while that of the other treated groups was stronger, which indicated the complexity of the mechanism of ~ (14) C action.