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目的为提高人骨髓成纤维细胞集落（ＣＦＵ－Ｆ）的产率，缩短培养时间。方法在以往的人骨髓基质细胞体外扩增方法基础上进行了改进，每１０ｍｌ培养体系中，含１×１０７个单个核细胞，以３０％人ＡＢ血清代替小牛血清，增加了碱性成纤维细胞生长因子（ｂＦＧＦ），再加入氢化可的松和适量的ＩＭＤＭ培养液，培养第９ｄ观察结果。结果在一定范围内人ＡＢ血清、ｂＦＧＦ均能促进ＣＦＵ－Ｆ增殖，提高其产率，并缩短培养时间３～５ｄ。结论可应用到人骨髓基质细胞体外扩增及传代中，对于研究ＣＦＵ－Ｆ的性质及在造血疾病中的应用具有重要意义
The aim is to improve the yield of human bone marrow fibroblast colonies (CFU-F) and shorten the culture time. Methods Based on the in vitro amplification methods of human bone marrow stromal cells, 1 × 107 mononuclear cells per 10 ml culture medium were used instead of bovine calf serum with 30% human AB serum, which increased basic fibroblasts Cell growth factor (bFGF), and then added hydrocortisone and the appropriate amount of IMDM culture medium, cultured 9d observation results. Results Within a certain range of human serum, bFGF can promote the proliferation of CFU-F, increase its yield and shorten the culture time of 3 ~ 5d. Conclusion It can be applied to the in vitro expansion and passage of human bone marrow stromal cells, which is of great significance for studying the properties of CFU-F and its application in hematopoietic diseases