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目的:研究逍遥散对D-半乳糖(D-gal)所致老年性痴呆(AD)模型小鼠学习记忆能力及血清超氧化物歧化酶(SOD)活性、脑组织丙二醛(MDA)含量的影响,探讨逍遥散防治AD的作用及其机制。方法:采用ip D-gal复制AD小鼠模型,同时用逍遥散全方组、疏肝健脾组、疏肝养血组、健脾养血组进行对抗治疗,共42 d。41 d进行学习能力训练,42 d进行记忆能力测试。然后取血及脑组织,测定小鼠血清SOD活性及脑组织MDA含量。结果:全方组、疏肝健脾组、疏肝养血组、健脾养血组均可提高小鼠学习记忆能力,降低潜伏时间,但以全方组最为明显;全方组、疏肝健脾组、疏肝养血组、健脾养血组皆可提高血清SOD活性,降低脑组织匀浆MDA含量,但亦以全方组最为明显。结论:逍遥散能较好的改善AD模型小鼠行为学指标,其可能与对抗自由基氧化有关。
OBJECTIVE: To study the effect of Xiaoyao powder on the learning and memory abilities and activities of serum superoxide dismutase (SOD) and malondialdehyde (MDA) in brain tissue of mice with senile dementia (AD) induced by D-galactose (AD). The influence of Xiaoyao San to prevent AD and its mechanism. Methods: AD mice model was duplicated with ip D-gal. At the same time, Xiaoyao Sanquan group, Shugan Jianpi group, Shugan Yangxue group and Jianpi Yangxue group were treated with antagonistic therapy for 42 days. 41 days learn ability training, 42 days memory test. Then blood and brain tissue were taken to determine the serum SOD activity and MDA content in the brain. Results: All groups, Shugan Jianpi group, Shugan Yangxue group, Jianpi Yangxue group can improve the learning and memory ability of mice, reduce the latency, but the most obvious in the whole group; the whole group, the liver The spleen-enhancing group, the liver-soothing and nourishing-blood group, and the spleen-nourishing and nourishing group all increased the serum SOD activity and decreased the brain tissue homogenate MDA content, but it was also most obvious in the whole group. Conclusion: Xiaoyao San can better improve the behavioral parameters of AD model mice, which may be related to the fight against free radical oxidation.